Antioxidant capacity is an overall ability of organisms or food to catch free radicals and prevent their harmful effect. Antioxidative effect includes protection of cells and cellular structures against harmful effect of free radicals, especially oxygen and nitrogen. Antioxidants are substances with antioxidative properties. They are most commonly present in fruits and vegetables. In addittion, other foods such as wine, meat or eggs contain these substances.

DMPD assay kit is recommended for antioxidant measurements of beverages. This kit measures the antioxidant activity of compounds that are able to transfer hydrogen atoms.

In our assay a solution of DMPD in the presence of a suitable oxidant solution, can form a stable and colored radical cation which shows an absorbance at 553 nm. Antioxidant compounds which are able to transfer a hydrogen atom to DMPD•+ quench the color and produce a discolorations of the solution which is proportional to their amount.