Fast ELISA kit for the quantitative determination of fumonisins in cereals and beer.

Overall process times were geared to requirements as defined by the USDA GIPSA test performance specifications.

Method: Competitive enzyme immunoassay

Detection limit: 15 ppb
Wheat (10 ppb) - Rye (25 ppb) - Barley (11 ppb) - Oats (15 ppb) - Corn (14 ppb) - Rice (25 ppb) - Beer (4 ppb)

Quantification limit: 50 ppb

Assay time: 20 min

Detection: 450 nm

Recovery rate:
Wheat (92%) - Rye (nd) - Barley (nd) - Oats (89%) - Corn (105%) - Rice (94%) - Beer (105%)
Standards: 0; 50; 150; 500; 1 500; 5 000 [ppb]

Ref. Range: 0 - 5 000 ppb

Storage temperature: 2 - 8ºC

Format: Microplate - 96 wells

- Quantitative results based on superior 4PL curve fitting for calibration
- Microsoft Excel-based calculation & evaluation tool available
- Reagents (excl. wash buffer) ready-to-use
- 96 well format with breakable microwell strips
- Simple extraction procedures
- Incubation at room temperature
- High accuracy and reproducibility
- High stability; minimum shelf life 12 months

Cereals and beer