Simple method for the measurement of phytic acid/total phosphorus in food and feed samples​

Shipping temperature: ambient

Storage temperature:
- Short term stability: 2-8^oC,
- Long term stability: see individual component labels

Assay format: spectrophotometer

Detection method: absorbance - 655

Signal response: increase

Linear range: ~ 0.5 to ~ 7.5 µg of phosphorus per assay

Reaction time: 25 min enzymic; 1 h for phosphate determination

Detection limit: 11,3 mg phosphorus (~ 40 mg phytic acid)

Stability: > 2 years under recommended storage conditions

 

• All reagents stable for > 2 years after preparation
• Spreadsheet available
• Standard included
• This method does not require purification of phytic acid via anion-exchange chromatography making it amenable to high numbers of samples
• Very cost effective

Seed materials, feeds and foodstuffs

Data Booklet Safety data sheet Data calculator Report validation

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