This sequential assay format reduces the manual input required by an analyst when compared to traditional lactose assay formats and therefore improves both accuracy and efficiency. When used in combination with the Creep Calculator provided, the β-galactosidase employed in this kit allows for the selective measurement of lactose in the presence of galacto-oligosaccharides (GOS) which are commonly found in lactose-free dairy products.
This constitutes a significant improvement over existing commercially available lactose assay kits which typically overestimate lactose content in lactose-free samples due to the unselective hydrolysis of GOS by β-galactosidase.
Lastly, the sensitivity of the K-LOLAC assay kit has been doubled through the use of a cascade biochemical pathway, helping to significantly reduce the LOD and LOQ for the assay.
British Patent Application No. 1710170.0.

Shipping temperature: ambient

Storage temperature:
- Short term stability: 2-8^oC,
- Long term stability: see individual component labels

Assay format: spectrophotometer

Detection method: absorbance - 340

Signal response: increase

Linear range: 1 to 50 µg of lactose (or 0.50 to 25 µg of D-glucose)

Reaction time: ~ 10 min

Detection limit: 1,62 mg/l

Stability: > 2 years under recommended storage condition

Method recognition: AOAC Method 2020.08

• World’s first sequential assay for lactose, i.e. improves accuracy and efficiency
• Contains a specific β-galactosidase for the selective measurement of lactose in dairy products
• Efficient pre-treatment step allows for accurate measurement of lactose in “low-lactose” and “lactose-free” dairy products
• Lower limit of detection (LOD) than any other commercially available enzymatic lactose detection method. LOD at 1,62 mg/L
• Very competitive price (cost per test)

Dairy products (milk, cream, whey powder, cheese, sweetened condensed milk ...), foods containing milk (dietary products, bakery, chocolate, confectionery), food additives, animal food, cosmetics, pharmaceuticals, samples, organic farming and infant feeding,..

Safety data sheet Data calculator Validation report K-LOLAC_Single_Lab_Validation Flyer K-LOLAC Data Booklet

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