Digestible and resistant starch - Enzymatic assay kit
Reference : K-DSTRS
The Digestible and Resistant Starch Assay Kit method is based on the research of Englyst et al. (Ref) with some modifications.
In line with Englyst definitions:
- Rapidly digestible starch (RDS) is that starch which is digested within 20 min.
- Slowly digestible starch (SDS) is that starch which is digested between 20 and 120 min.
- A new term, ‘Total digestible starch (TDS)’ is introduced (and measured) to cover all starch that is digested within 4 h (the average time of residence of food in the human small intestine).
- Resistant starch (RS) then, is that starch which is not digested within 4 h.
Storage temperature:
- Short term stability: 2-8oC,
- Long term stability: see individual component labels
Assay format: spectrophotometer, microplate
Detection method: absorbance - 510
Signal response: increase
Linear range: 5 to 100 µg of D-glucose per assay
Total assay time: ~ 360 min
Detection limit: 2 g/100 g
Stability: > 2 years under recommended storage conditions
- The incubation conditions parallel those used in AOAC Method 2017.16, a new, rapid integrated procedure for the measurement of total dietary fiber (K-RINTDF).
- This method is physiologically based and designed to fit the definition of DF announced by Codex Alimentarius in 2009
- Digestion is performed using saturating levels of pancreatic α-amylase (PAA) and amyloglucosidase (AMG), but in stirred containers rather than shaken tubes, to simplify sample removal
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Specifications
Reference | Packaging | |
---|---|---|
K-DSTRS | 40 tests de chaque (of each) |