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Malt β-glucanase / Lichenase - Enzyme activity assay kit
Reference : K-MBG4
Method for the measurement of Malt β-glucanase / Lichenase
Countries eligible for delivery
Countries eligible for delivery:
France (except Corsica and overseas departments and territories), Algeria, French overseas departments and territories, French Polynesia, Libyan Arab Jamahiriya, Morocco, Tunisia, Egypt, France (Corsica)
Method for the measurement of Malt β-glucanase / Lichenase
Shipping temperature: ambient
Storage temperature:
- Short term stability: 2-8oC,
- Long term stability: see individual component labels
Assay format: spectrophotometer, auto-analyser
Detection method: absorbance - 400
Signal response: increase
Total assay time:
Malt β-glucanase: 20 min (manual) / 10 min (auto-analyser)
Lichenase: 10 min (manual) / 10 min (auto-analyser)
Detection limit:
- Malt β-glucanase : 4,3 x 10-4 U/mL
- Lichenase : 9,1 x 10-5 U/ml
Reproducibility (%): ~ 3%
Stability: > 2 years under recommended storage conditions
The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(31-β-D-cellotriosyl-glucoside) (BCNPBG4).
The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase.
Mixed linkage β-glucanase (endo-1,3:1,4-β-glucanase) / Lichenase (EC 3.2.1.73) acts specifically to release 2-chloro-4-nitrophenol (CNP) from this substrate. The rate of release of CNP is directly related to the β-glucanase/Lichenase activity in a sample. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).
NOTE: the substrate is not hydrolysed by β-glucosidase or cellobiohydrolase. The substrate can be hydrolysed by certain endo-cellulases (e.g. Trichoderma sp.) but this does not result in an increase in absorbance.
Novel method
Shipping temperature: ambient
Storage temperature:
- Short term stability: 2-8oC,
- Long term stability: see individual component labels
Assay format: spectrophotometer, auto-analyser
Detection method: absorbance - 400
Signal response: increase
Total assay time:
Malt β-glucanase: 20 min (manual) / 10 min (auto-analyser)
Lichenase: 10 min (manual) / 10 min (auto-analyser)
Detection limit:
- Malt β-glucanase : 4,3 x 10-4 U/mL
- Lichenase : 9,1 x 10-5 U/ml
Reproducibility (%): ~ 3%
Stability: > 2 years under recommended storage conditions
The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(31-β-D-cellotriosyl-glucoside) (BCNPBG4).
The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase.
Mixed linkage β-glucanase (endo-1,3:1,4-β-glucanase) / Lichenase (EC 3.2.1.73) acts specifically to release 2-chloro-4-nitrophenol (CNP) from this substrate. The rate of release of CNP is directly related to the β-glucanase/Lichenase activity in a sample. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).
NOTE: the substrate is not hydrolysed by β-glucosidase or cellobiohydrolase. The substrate can be hydrolysed by certain endo-cellulases (e.g. Trichoderma sp.) but this does not result in an increase in absorbance.
Novel method
- Very cost effective
- All reagents stable for > 2 years
- Specific for endo-1,3:1,4-β-glucanase/Lichenase
- Simple, convenient, rapid assay
- Suitable for manual and auto-analyzer
- Malt flour standard and lichenase standard included
Crude malt extracts and enzyme preparations
Safety data sheet
Data calculator Malt beta-glucanase
Data calculator Lichenase
Data Booklet
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Specifications
| Reference | Packaging | |
|---|---|---|
| K-MBG4 | 100 tests (manuel/manual) / 400 tests (automate/auto-analyser) |