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Endo-xylanase (XylX6 method) - Enzyme activity assay kit
Reference : K-XylX6
Method for the measurement of endo-xylanase (XylX6 method)
Countries eligible for delivery
Countries eligible for delivery:
France (except Corsica and overseas departments and territories), Algeria, French overseas departments and territories, French Polynesia, Libyan Arab Jamahiriya, Morocco, Tunisia, Egypt, France (Corsica)
Method for the measurement of endo-xylanase (XylX6 method)
Shipping temperature: ambient
Storage temperature:
- Short term stability: 2-8oC,
- Long term stability: see individual component labels
Assay format: spectrophotometer, auto-analyser
Detection method: absorbance - 400 ; 405
Signal response: increase
Total assay time: 10 min
Detection limit: 5,3 x 10-4 U/ml
Reproducibility (%): ~ 3%
Stability: > 2 years under recommended storage conditions
The XylX6 assay reagent contains two components:
1) 4,6-O-(3-ketobutylidene)-4-nitrophenyl-β-D-45-glucosyl-xylopentaoside
2) β-xylosidase
The ketone blocking group prevents any hydrolytic action by the β-xylosidase or other exo-acting glycosidases on the XylX6 substrate. Incubation with an endo-xylanase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-Xxylosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of XylX6 by the endo-xylanase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10,0).
Note that standard curves relating the absorbance obtained using the XylX6 assay to endo-xylanase activity on the native substrates, wheat arabinoxylan and beechwood xylan, are provided in the Supporting Information file under the Documentation tab.
Novel method
Shipping temperature: ambient
Storage temperature:
- Short term stability: 2-8oC,
- Long term stability: see individual component labels
Assay format: spectrophotometer, auto-analyser
Detection method: absorbance - 400 ; 405
Signal response: increase
Total assay time: 10 min
Detection limit: 5,3 x 10-4 U/ml
Reproducibility (%): ~ 3%
Stability: > 2 years under recommended storage conditions
The XylX6 assay reagent contains two components:
1) 4,6-O-(3-ketobutylidene)-4-nitrophenyl-β-D-45-glucosyl-xylopentaoside
2) β-xylosidase
The ketone blocking group prevents any hydrolytic action by the β-xylosidase or other exo-acting glycosidases on the XylX6 substrate. Incubation with an endo-xylanase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-Xxylosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of XylX6 by the endo-xylanase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10,0).
Note that standard curves relating the absorbance obtained using the XylX6 assay to endo-xylanase activity on the native substrates, wheat arabinoxylan and beechwood xylan, are provided in the Supporting Information file under the Documentation tab.
Novel method
- Very cost effective
- All reagents stable for > 4 years
- Completely specific for endo-1,4-β-xylanase
- Generally applicable and highly sensitive
- Simple format
- Suitable for manual and auto-analyzer
- Excellent reproducibility
- Standard included
Enzyme extracts, fermentation broths or commercial enzyme preparations
Safety data sheet
Validation report
Data calculator
Supporting information
Data Booklet
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Specifications
| Reference | Packaging | |
|---|---|---|
| K-XylX6-2V | 200 tests (manuel/manual), 400 tests (automate/auto-analyser) |