Enzyme Pullulanase M1

Shipping temperature: ambient

Storage temperature: 2-8^oC

Formulation: in 3,2 M ammonium sulphate

Physical form: suspension

EC 3.2.1.41
CAZy Family: GH13
CAS: 9075-68-7

Synonyms: pullulanase; pullulan 6-α-glucanohydrolase

Source: Klebsiella planticola
Highly purified

Molecular weight: 109,000

Concentration: supplied at ~ 650 U/mL

Specific activity:
~ 30 U/mg (40°C, pH 5,0 on pullulan)

Unit definition:
One Unit of pullulanase M2 activity is defined as the amount of enzyme required to release one micromole of glucose reducing-sugar-equivalents per minute from pullulan (5 mg/mL) in sodium acetate buffer (100 mM), pH 5,0 at 40°C

Specificity:
Hydrolysis of (1,6)-α-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the α- and β-limit dextrins of amylopectin and glycogen

Temperature optima: 40°C
pH optima: 5

Stability: > 4 years at 4°C

• High purity and stability

- Research, biochemical enzyme assays and in vitro diagnostic analysis
- Recommended pullulanase for research on starch structure
- Cereals, food and feeds industries particularly in starch saccharification and production of high glucose or maltose syrups.
 

Data Booklet Safety data sheet

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