Protease (Subtilisin A from Bacillus licheniformis)
Reference : E-BSPRT
CAS Number : 9014-01-1
Shipping temperature: ambient
Storage temperature: 2-8oC
Formulation: in 3,2 M ammonium sulphate
Physical form: suspension
EC 3.4.21.62
CAS: 9014-01-1
Synonyms: subtilisin; subtilisin A
Source: Bacillus licheniformis
Highly purified
Molecular weight: 30,250
Specific activity:
~ 6 U/mg of protein (40°C, pH 8,0 on casein)
Specificity:
Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1;
Hydrolyses peptide amides
Unit definition:
One unit will hydrolyse casein to produce colour equivalent to one micromole (181 µg) of tyrosine per minute at pH 7,0 at 40°C (colour by Folin-Ciocalteu reagent)
Temperature optima: 60°C
pH optima: 7
Stability: > 4 years at 4°C
E-BSPRT-A-100ML specifically to be used with ANKOMTDF Dietary Fiber Analyzer
- High purity and stability
- Enzyme recommended for use in the Total Dietary Fiber test method and AOAC INTERNATIONAL Total Dietary Fibre analytical procedures
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Specifications
Reference | Packaging | |
---|---|---|
E-BSPRT-40ML | 2 g (40 mL) | |
E-BSPRT-100ML | 5 g (100 mL) | |
E-BSPRT-A-100ML | 2,5g (100 mL) - pour analyseur des fibres totales ANKOM |