Enzyme Phosphotransacetylase

Shipping temperature: ambient

Storage temperature: 2-8^oC

Formulation: in 3,2 M ammonium sulphate

Physical form: suspension

EC 2.3.1.8
CAS: 9029-91-8

Synonyms: phosphate acetyltransferase; acetyl-CoA:phosphate acetyltransferase

Source: Bacillus subtilis
​Recombinant from Bacillus subtilis

Molecular weight: 36,953

Concentration: supplied at ~ 3,000 U/mL

Specific activity:
~ 13,000 U/mg (25°C, pH 7,4 on Coenzyme A)

Unit definition:
One Unit of phosphotransacetylase activity is defined as the amount of enzyme required to release one micromole of acetyl-CoA per minute from Coenzyme A (0,44 mM) in Tris.HCl buffer (86 mM) pH 7,4 at 25°C

Specificity:
Catalyses the reaction:
Coenzyme A + acetyl phosphate = acetyl-CoA + phosphate

Temperature optima: 25°C
pH optima: 7,4

Stability: > 2 years at 4°C

• High purity and stability

- Research, biochemical enzyme assays and in vitro diagnostic analysis
- Measurement of acetic acid in the food, fermentation, wine, beverage and dairy industries

Data Booklet Safety data sheet

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