Enzyme Pectate lyase

Shipping temperature: ambient

Storage temperature: <-10°C

Formulation: in 50% (v/v) glycerol

Physical form: solution

EC 4.2.2.2
CAZy Family: PL1
CAS: 9015-75-2

Synonyms: pectate lyase; -α-D-galacturonan lyase

Source: Aspergillus sp.
​Highly purified

Molecular weight: 45,000

Concentration: supplied at ~ 1,400 U/mL

Specific activity:
~ 180 U/mg (40°C, pH 8.0 on polygalacturonic acid)

Unit definition:
One Unit of pectate lyase activity is defined as the amount of enzyme required to release one micromole of galacturonic acid from polygalacturonic acid (2.5 mg/mL) per min in Tris.HCl buffer (50 mM), pH 8,0 at 40°C

Specificity:
Eliminative cleavage of (1,4)-α-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends

Temperature optima: 55°C
pH optima: 8

Stability: > 4 years at 4°C or - 10°C

• High purity and stability

- Research, biochemical enzyme assays and in vitro diagnostic analysis
- Suitable for pectin identification according to USP method [Pectin 9000-69-5]; Pectin Identification Procedure. Applications in carbohydrate and biofuels research

Data Booklet Safety data sheet

The documents available in this section are provided for information only and are subject to change. Please contact us for the latest updates.

If you have just received a kit or product without technical instructions in paper format, this means that the instructions have been dematerialized.
In this case, please download it and refer only to the version you can download from the product page of the manufacturer's website, or by scanning the QR code present on the product.

Regarding any certificates of analysis, safety data sheets or spreadsheets, please contact us to obtain the latest versions.

If you have any questions, our team is at your disposal.