Enzyme Isoamylase (Glycogen 6-glucanohydrolase)

Shipping temperature: ambient

Storage temperature: 2-8^oC

Formulation: in 3,2 M ammonium sulphate

Physical form: suspension

EC 3.2.1.68
CAZy Family: GH13
CAS: 9067-73-6

Synonyms: isoamylase; glycogen 6-α-D-glucanohydrolase

Source: Pseudomonas sp.
​Highly purified

Molecular weight: 71,500

Concentration: supplied at ~ 200 U/mL

Specific activity:
~ 180 U/mg (40°C, pH 4,0 on oyster glycogen)(equivalent to 16 MU Sigma U/mg)

Unit definition:
One unit of isoamylase activity is the amount of enzyme required to release one μmole of D-glucose reducing sugar equivalent in the presence of oyster glycogen per min at pH 4,0 and 40°C

Specificity:
Hydrolysis of (1,6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins

Temperature optima: 50°C
pH optima: 4

Method recognition: AOAC method 2000.11 and GB standard 5009.245-2016

Stability:> 4 years at 4°C

• High purity and stability

- Research, biochemical enzyme assays and in vitro diagnostic analysis
- Carbohydrate research and in the food and feeds, and cereals industry

Data Booklet Safety data sheet

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