Enzyme Hexokinase/Glucose-6-phosphate dehydrogenase

Shipping temperature: ambient

Storage temperature: 2-8^oC

Formulation: in 3,2 M ammonium sulphate

Physical form: suspension

EC:
- Hexokinase: 2.7.1.1
- Glucose-6-phosphate dehydrogenase: 1.1.1.49

CAS:
- Hexokinase: 9001-51-8
- Glucose-6-phosphate dehydrogenase: 9001-40-5

Synonyms:
- Hexokinase: hexokinase; ATP:D-hexose 6-phosphotransferase
- Glucose-6-phosphate dehydrogenase: glucose-6-phosphate dehydrogenase (NADP+); D-glucose-6-phosphate:NADP+ 1-oxidoreductase

Source:
- Hexokinase: recombinant from yeast
- G6P-DH: recombinant from Leuconostoc mesenteroides

Activity/Concentration:
- Hexokinase: ~ 420 U/mL
- G6P-DH: ~ 210 U/mL

Unit definition:
- Hexokinase: one unit of hexokinase activity is defined as the amount of enzyme required to produce one micromole of NADH from NAD+ in the presence of D-glucose and glucose-6-phosphate dehydrogenase at pH 7.4 and 25°C.
- Glucose-6-phosphate dehydrogenase: one unit of Glucose-6-phosphate dehydrogenase activity is the amount of enzyme required to convert one micromole of glucose-6-phosphate to 6-phosphogluconate per minute, in the presence of NADP+ at pH 7.4 and 25°C

Specificity:
- Hexokinase:
Catalyses the reaction:
ATP + D-hexose = ADP + D-hexose 6-phosphate
Phopshorylates D-glucose, D-mannose, D-fructose, sorbitol and D-glucosamine.
- Glucose-6-phosphate dehydrogenase:
Catalyses the reaction:
D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+

Stability: > 4 years at 4°C

• High purity and stability

- Research, biochemical enzyme assays and in vitro diagnostic analysis
- Measurement of glucose and other hexoses in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries

Data Booklet Safety data sheet

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