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Galactose dehydrogenase - Galactose mutarotase

Reference : E-GALMUT

CAS Number : 9028-54-0

Enzyme Galactose dehydrogenase - Galactose mutarotase
Countries eligible for delivery
Countries eligible for delivery:
France (except Corsica and overseas departments and territories), Algeria, French overseas departments and territories, French Polynesia, Libyan Arab Jamahiriya, Morocco, Tunisia, Egypt, France (Corsica)
Enzyme Galactose dehydrogenase - Galactose mutarotase

Shipping temperature: ambient

Storage temperature: 2-8oC

Formulation: in 3,2 M ammonium sulphate

Physical form: suspension

EC number :
Galactose dehydrogenase: 1.1.1.48
Galactose mutarotase: 5.1.3.3
CAS:
Galactose dehydrogenase: 9028-54-0
Galactose mutarotase: 9031-76-9

Synonyms:
Galactose dehydrogenase: D-galactose 1-dehydrogenase; D-galactose:NAD+ 1-oxidoreductase
Galactose mutarotase: aldose 1-epimerase; aldose 1-epimerase

Source: Escherichia coli

Specific activity:
Galactose dehydrogenase: ~ 200 U/mL at pH 8,6 and 25oC
Galactose mutarotase: 4,1 mg/mL

Specificity:
Interconversion of the α- and β-anomeric forms of D-galactose is catalysed by β-galactose mutarotase (GMR) (1). 

(1) α-D-Galactose ↔ β-D-Galactose.

The β-D-Galactose is oxidised by NAD+ to D-galactonic acid in the presence of β-galactose dehydrogenase (GALDH) at pH 7.5 (2).

(2) β-D-Galactose + NAD+ → D-galactonic acid + NADH + H

Unit definition: one unit of galactose dehydrogenase is defined as the amount of enzyme required to produce one µmole of NADH from NAD+ per minute at pH 8,6, at 25°C

Temperature optima: 25°C
pH optima: 8,6

Stability: minimun 1 year at 4°C
  • High purity and stability
- Research, biochemical enzyme assays and in vitro diagnostic analysis
- Measurement of D-galactose or L-arabinose in the L-Arabinose/D-Galactose Assay Kit (K-ARGA).
- Measurement of lactose via d-galactose in the Lactose/D-Galactose (Rapid) Assay Kit (K-LACGAR).
Data Booklet Safety data sheet


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Specifications

Reference Packaging  
E-GALMUT 1 mL - Dehydrogénase 200 U/mL; Mutarotase 4 mg/mL
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