Enzyme Fructanase mixture

Shipping temperature: ambient

Storage temperature: <-10°C

Formulation: supplied as a lyophilised powder

Physical form: powder

EC Number:
- Endo-inulinase: 3.2.1.7
- Exo-inulinase: 3.2.1.80
CAZy Family: GH32
CAS Number: 9001-57-4 / 9025-67-6 / 37288-56-5

Synonyms:
- Endo-inulinase: 1-β-D-fructan fructanohydrolase
- Exo-inulinase: fructan β-fructosidase

Source: Aspergillus sp.

Specificity:    
- Endo-inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin
- Exo-inulinase: hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides

Unit definition:
- Endo-Inulinase: one unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4,5
- Exo-inulinase: one unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4,5 at 40°C

Components: exo-inulinase 20,000 U (on kestose, at 40°C), endo-inulinase 1000 U (on fructan at 40°C), α-galactosidase 0,70 U (on p-nitrophenyl α-galactoside at 40°C), β-glucanase 1.25 U (on β-glucan at 40°C) and pectinase 5,5 U (on pectin at 40°C)

Note: This product has been purified to remove most of the contamination with α-galactosidase, β-glucanase and pectinase which can interfere with the use of the preparation in the measurement of fructan, or in the solubilisation of "insoluble" fructan in the AOAC Total Dietary Fiber method. In this product, the contamination of exo-inulinase by α-galactosidase, β-glucanase and pectinase is 0,003%, 0,005% and 0,022%, respectively. In Fructozyme (NOVO), the contamination by alpha-galactosidase, β-glucanase and pectinase is 15,2%, 0,3% and 2% respectively.

Temperature optima: 40°C
pH optima: 4,5

Method recognition: AOAC method 997.08, AOAC method 2000.11, AOAC method 2016.06 and GB standards 5009.255-2016

Stability: minimum 1 year at < -10°C

• High purity and stability

Data Booklet Safety data sheet

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