Enzyme Exo-1,3-β-D-glucanase + β-glucosidase

Shipping temperature: ambient

Storage temperature: 2-8^oC

Formulation: in 3,2 M ammonium sulphate

Physical form: suspension

EC Number:
- Exo-1,3-β-glucanase: 3.2.1.58
- β-glucosidase: 3.2.1.21
CAZy Family: GH3, GH55
CAS:
- Exo-1,3-β-glucanase: 9073-49-8
- β-glucosidase: 9001-22-3

Synonyms:
- Exo-1,3-β-glucanase: glucan 1,3-β-glucosidase; 3-β-D-glucan glucohydrolase
- β-glucosidase: β-glucosidase

Source:   
- Exo-1,3-β-glucanase: Trichoderma virens
- β-glucosidase: Aspergillus niger

Activity/Concentration:
Exo-1,3-β-glucanase (100 U/mL) / β-glucosidase (20 U/mL)

Unit definition:
- One Unit of exo-1,3-β-glucanase activity is defined as the amount of enzyme required to release one micromole of glucose reducing-sugar equivalents per minute from laminarin (10 mg/mL) in sodium acetate buffer (100 mM), pH 4,0 at 40°C
- One Unit of β-glucosidase activity is defined as the amount of enzyme required to release one micromole of p-nitrophenol per minute from 4-nitrophenyl-β-D-glucpyranoside in sodium acetate buffer (100 mM), pH 4,0 at 40°C

Specificity:
- Exo-1,3-β-glucanase: Successive hydrolysis of β-D-glucose units from the non-reducing ends of (1,3)-β-D-glucans, releasing α-glucose
- β-glucosidase: Hydrolysis of terminal, non-reducing β-D-glucosyl residues with release of β-D-glucose

Température optimale : 40°C
pH optimal : 4

Stability: minimum 1 year at 4^oC

• High purity and stability

- Research, biochemical enzyme assays and in vitro diagnostic analysis
- Food and feeds, carbohydrate and biofuels industries

Data Booklet Safety data sheet

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