Enzyme Chitinase

Shipping temperature: ambient

Storage temperature: 2-8^oC

Formulation: in 3,2 M ammonium sulphate

Physical form: suspension

EC3.2.1.14
CAZy Family: GH18
CAS: 9001-06-3

Synonyms: chitodextrinase; 1,4-β-poly-N-acetylglucosaminidase

Source: Clostridium thermocellum
Recombinant from Clostridium thermocellum

Molecular weight: 53,000

Concentration: supplied at ~ 3 U/mL

Specific activity:
~ 0,3 U/mg (40°C, pH 6,2 onp-nitrophenyl-β-triacetylchitotriose)

Unit definition:
One Unit of chitinase activity is defined as the amount of enzyme required to release one micromole ofp-nitrophenol per minute fromp-nitrophenyl-beta-triacetylchitotriose (2,5 mM) in MES buffer (100 mM), pH 6,2 at 40°C

Specificity:
Random hydrolysis of N-acetyl-β-D-glucosaminide (1-4)-β-linkages in chitin and chitodextrins

Temperature optima: 40°C
pH optima: 6,2

Stability: > 4 years at 4°C

• High purity and stability

- Research, biochemical enzyme assays and in vitro diagnostic analysis

Data Booklet Safety data sheet

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