Succinyl-CoA synthetase (prokaryote)
Reference : E-SCOAS
CAS Number : 9080-33-5
Shipping temperature: ambient
Storage temperature: 2-8oC
Formulation: in 3,2 M ammonium sulphate
Physical form: suspension
EC 6.2.1.5
CAS: 9080-33-5
Synonyms: succinate-CoA ligase (ADP-forming); succinate:CoA ligase (ADP-forming)
Source: prokaryotic
Molecular weight: 30,843 / 41,393
Concentration: supplied at ~ 220 U/mL
Specific activity:
~ 8 U/mg (25°C, pH 8,4 on succinic acid)
Unit definition:
One Unit of Succinyl-CoA synthetase activity is defined as the amount of enzyme required to release one micromole of succinyl CoA from succinic acid (5,8 mM) per minute in the presence of NADH and Coenzyme A in Glycylglycine buffer (34 mM), pH 8,4 at 25°C
Specificity:
Catalyses the reaction:
ATP + succinate + CoA = ADP + phosphate + succinyl-CoA
Temperature optima: 25°C
pH optima: 8,4
Stability: > 2 years at 4°C
- High purity and stability
- Measurement of succinic acid in the clinical chemistry, food, wine and dairy industries
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Specifications
Reference | Packaging | |
---|---|---|
E-SCOAS | 550 unités (units) |