Enzyme Exo-α-sialidase

Shipping temperature: ambient

Storage temperature: 2-8^oC

Formulation: in 20 mM tris buffer containing 50 mM NaCl2 and 5 mM EDTA

Physical form: suspension

EC 3.2.1.18
CAZy Family: GH33
CAS: 9001-67-6

Synonyms: exo-α-sialidase; acetylneuraminyl hydrolase

Source: Clostridium perfringens

Molecular weight: 43,600

Concentration: supplied at ~ 500 U/mL

Specific activity:
~ 140 U/mg (37°C, pH 7.0 on pNP-α-D-N-acetylneuraminic acid)

Unit definition:
One unit of sialidase activity is defined as the amount of enzyme required to release one micromole of p-nitrophenol per minute from pNP-alpha-D-N-acetylneuraminic acid (1 mM) in sodium phosphate buffer (100 mM), pH 7,0 at 37°C

Specificity:
Hydrolysis of unbranched, non-reducing terminal α-2,3-linked, α-2,6-linked >> α-2,8-linked N-acetylneuraminic acid (NANA; Neu5Ac) residues from glycoproteins and oligosaccharides of glycoconjugates

Temperature optima: 37°C
pH optima: 7

Stability: >2 years at 4°C

• High purity and stability

- Research, biochemical enzyme assays and in vitro diagnostic analysis
- Clinical chemistry, glycobiology and carbohydrate research

Data Booklet Safety data sheet

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