3-Hydroxybutyrate dehydrogenase (prokaryote)

Reference : E-HBDH

CAS Number : 9028-38-0

Enzyme 3-Hydroxybutyrate dehydrogenase
Countries eligible for delivery
Countries eligible for delivery:
France (except Corsica and overseas departments and territories), Algeria, French overseas departments and territories, French Polynesia, Libyan Arab Jamahiriya, Morocco, Tunisia, Egypt, France (Corsica)
Enzyme 3-Hydroxybutyrate dehydrogenase

Shipping temperature
: 
ambient

Storage temperature
:
 2-8oC

Formulation: in 3,2 M ammonium sulphate

Physical form: suspension

EC 1.1.1.30
CAS:
9028-38-0

Synonym3-hydroxybutyrate dehydrogenase; (R)-3-hydroxybutanoate:NAD+ oxidoreductase

Source: prokaryote

Molecular weight: 29,177

Concentrationsupplied at ~ 100 U/mL

Expression: recombinant from a prokaryotic source

Specific activity:
77,4 U/mg (25°C, pH 8,0 on D-β-hydroxybutyric acid)

Unit definition:
One Unit of 3-hydroxybutyrate dehydrogenase is defined as the amount of enzyme required to oxidise one micromole of D-β-hydroxybutyric acid (28,3 mM) per minute in the presence of NAD+ in Tris-HCl buffer (143 mM), pH 8,0 at 25°C

Specificity:
Catalyses the reaction:
D-3-Hydroxybutyrate + NAD+ = acetoacetate + NADH + H+

Temperature optima25°C
pH optima: 8

Stability: > 2 years at 4°C
  • High purity and stability
- Research, biochemical enzyme assays and in vitro diagnostic analysis 
- Food, fermentation, wine, beverage and dairy industries
Data Booklet Safety data sheet


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Specifications

Reference Packaging  
E-HBDH 200 unités (units)