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3-Hydroxybutyrate dehydrogenase (prokaryote)
Reference : E-HBDH
CAS Number : 9028-38-0
Enzyme 3-Hydroxybutyrate dehydrogenase
Countries eligible for delivery
Countries eligible for delivery:
France (except Corsica and overseas departments and territories), Algeria, French overseas departments and territories, French Polynesia, Libyan Arab Jamahiriya, Morocco, Tunisia, Egypt, France (Corsica)
Enzyme 3-Hydroxybutyrate dehydrogenase
Shipping temperature: ambient
Storage temperature: 2-8oC
Formulation: in 3,2 M ammonium sulphate
Physical form: suspension
EC 1.1.1.30
CAS: 9028-38-0
Synonym: 3-hydroxybutyrate dehydrogenase; (R)-3-hydroxybutanoate:NAD+ oxidoreductase
Source: prokaryote
Molecular weight: 29,177
Concentration: supplied at ~ 100 U/mL
Expression: recombinant from a prokaryotic source
Specific activity:
77,4 U/mg (25°C, pH 8,0 on D-β-hydroxybutyric acid)
Unit definition:
One Unit of 3-hydroxybutyrate dehydrogenase is defined as the amount of enzyme required to oxidise one micromole of D-β-hydroxybutyric acid (28,3 mM) per minute in the presence of NAD+ in Tris-HCl buffer (143 mM), pH 8,0 at 25°C
Specificity:
Catalyses the reaction:
D-3-Hydroxybutyrate + NAD+ = acetoacetate + NADH + H+
Temperature optima: 25°C
pH optima: 8
Stability: > 2 years at 4°C
Shipping temperature: ambient
Storage temperature: 2-8oC
Formulation: in 3,2 M ammonium sulphate
Physical form: suspension
EC 1.1.1.30
CAS: 9028-38-0
Synonym: 3-hydroxybutyrate dehydrogenase; (R)-3-hydroxybutanoate:NAD+ oxidoreductase
Source: prokaryote
Molecular weight: 29,177
Concentration: supplied at ~ 100 U/mL
Expression: recombinant from a prokaryotic source
Specific activity:
77,4 U/mg (25°C, pH 8,0 on D-β-hydroxybutyric acid)
Unit definition:
One Unit of 3-hydroxybutyrate dehydrogenase is defined as the amount of enzyme required to oxidise one micromole of D-β-hydroxybutyric acid (28,3 mM) per minute in the presence of NAD+ in Tris-HCl buffer (143 mM), pH 8,0 at 25°C
Specificity:
Catalyses the reaction:
D-3-Hydroxybutyrate + NAD+ = acetoacetate + NADH + H+
Temperature optima: 25°C
pH optima: 8
Stability: > 2 years at 4°C
- High purity and stability
- Research, biochemical enzyme assays and in vitro diagnostic analysis
- Food, fermentation, wine, beverage and dairy industries
- Food, fermentation, wine, beverage and dairy industries
Data Booklet
Safety data sheet
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Specifications
| Reference | Packaging | |
|---|---|---|
| E-HBDH | 200 unités (units) |