ELISA kit for the quantitative determination of total aflatoxins in food samples.

Overall process times were geared to requirements as defined by the USDA GIPSA test performance specifications.

Method: Competitive enzyme immunoassay

Detection limit: 0.6 ppb
0.8 (Wheat) - 1.2 (Oats) - 1.5 (Barley) - 1.2 (Rice) - 1.3 (Corn) - 1.2 (Beer) - 1.3 (Peanut) - 1 , 5 (Pistache) - 1,2 (DDGS *) - 1,2 (Grape) - 1,3 (Hazelnut) - 1,1 (Cotton seed)
* Distillers Dried Grains with Solubles

Quantification limit: 2.1 ppb
2.8 (Wheat) - 3.7 (Oats) - 3.0 (Barley) - 2.8 (Rice) - 2.2 (Corn) - 2.4 (Beer) - 2.5 (Peanut) - 3 , 5 (Pistache) - 3.0 (DDGS *) - 3.6 (Raisin) - 2.0 (Hazelnut) - 1.8 (Cotton seed)

Assay time: 10 min

Detection: 450 nm

Recovery rate:
- Artificially contaminated samples: 82 - 100%
- Naturally contaminated samples: 92 - 113%

Standards: 0; 2; 5; 10; 20; 50 ppb

Cross reaction:
- Aflatoxin B1 110%
- Aflatoxin B2 74%
- Aflatoxin G1 69%
- Aflatoxin G2 47%


Storage temperature: 2 - 8ºC

Format: Microplate - 96 wells

- Quantitative results based on superior 4PL curve fitting for calibration
- Microsoft Excel-based calculation & evaluation tool available
- Reagents (excl. wash buffer) ready-to-use
- 96 well format with breakable microwell strips
- Simple extraction procedures
- Incubation at room temperature
- High accuracy and reproducibility
- High stability; long shelf life

Cereals and beer