Fast ELISA kit for the quantitative determination of aflatoxin B1 in cereals and beer.

Overall process times were geared to requirements as defined by the USDA GIPSA test performance specifications.

Method: Competitive enzyme immunoassay

Detection limit: 0.5 ppb
Wheat (0.7 ppb) - Rye (1.1 ppb) - Barley (0.9 ppb) - Oats (0.7 ppb) - Corn (1.4 ppb) - Rice (1.1 ppb) - Beer ( 0.9 ppb)

Quantification limit: 1,5 ppb

Assay time: 20 min

Detection: 450 nm

Recovery rate:
Wheat (104%) - Rye (103%) - Barley (98%) - Oats (86%) - Corn (94%) - Rice (91%) - Beer (114%)

Standards: 0; 1,5; 3; 6; 12; 24 [ppb]

Ref. range: 0 - 24 ppb

Cross reaction:
- Aflatoxine B2 29%
- Aflatoxine G1 44%
- Aflatoxine G2 5%
- Aflatoxine M1 2%

Storage temperature: 2 - 8ºC

Format: Microplate - 96 wells

- Quantitative results based on superior 4PL curve fitting for calibration
- Microsoft Excel-based calculation & evaluation tool available
- Reagents (excl. wash buffer) ready-to-use
- 96 well format with breakable microwell strips
- Simple extraction procedures
- Incubation at room temperature
- High accuracy and reproducibility
- High stability; minimum shelf life 12 months

Cereals and beer