Fast ELISA kit for the quantitative determination of zearalenone in cereals and beer.

Overall process times were geared to requirements as defined by the USDA GIPSA test performance specifications.

Method: Competitive enzyme immunoassay

Detection limit: 5 ppb
Wheat (7 ppb) - Rye (8 ppb) - Barley (8 ppb) - Oats (8 ppb) - Corn (8 ppb) - Rice (7 ppb) - Beer (7 ppb)

Quantification limit: 10 ppb

Assay time: 20 min

Detection:  450 nm

Recovery rate:
Wheat (90%) - Barley (nd) - Rye (nd) - Oats (101%) - Rice (99%) - Corn (99%) - Beer (100%)
Standards: 0; 10; 25; 75; 200; 500 [ppb]

Ref. Range: 0 - 5 00 ppb

Cross reaction:
- β-Zearalanol 17%
- α-Zearalenol 73%
- β-Zearalenol 23 %


Storage temperature: 2 - 8ºC

Format: Microplate - 96 wells

- Quantitative results based on superior 4PL curve fitting for calibration
- Microsoft Excel-based calculation & evaluation tool available
- Reagents (excl. wash buffer) ready-to-use
- 96 well format with breakable microwell strips
- Simple extraction procedures
- Incubation at room temperature
- High accuracy and reproducibility
- High stability; minimum shelf life 12 months

Cereals and beer

Flyer Kit ELISA mycotoxines LIBIOS 2019.pdf

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