Enzyme Succinyl-CoA synthetase

Shipping temperature: ambient

Storage temperature: 2-8^oC

Formulation: in 3,2 M ammonium sulphate

Physical form: suspension

EC 6.2.1.5
CAS: 9080-33-5

Synonyms: succinate-CoA ligase (ADP-forming); succinate:CoA ligase (ADP-forming)

Source: prokaryotic

Molecular weight: 30,843 / 41,393

Concentration: supplied at ~ 220 U/mL

Specific activity:
~ 8 U/mg (25°C, pH 8,4 on succinic acid)

Unit definition:
One Unit of Succinyl-CoA synthetase activity is defined as the amount of enzyme required to release one micromole of succinyl CoA from succinic acid (5,8 mM) per minute in the presence of NADH and Coenzyme A in Glycylglycine buffer (34 mM), pH 8,4 at 25°C

Specificity:
Catalyses the reaction:
ATP + succinate + CoA = ADP + phosphate + succinyl-CoA

Temperature optima: 25°C
pH optima: 8,4

Stability: > 2 years at 4°C

• High purity and stability

- Research, biochemical enzyme assays and in vitro diagnostic analysis
- Measurement of succinic acid in the clinical chemistry, food, wine and dairy industries

Data Booklet Safety data sheet

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